Basic concepts of tissue and cell engineering are taught by faculty of Medicine and Engineering, Chulalongkorn University. Principles of generation of tissue and organ including cell sources, scaffolds and culture condition are described. Cell-extracellular matrix interaction and environmental factors (e.g. growth factors, soluble factors, mechanical loading) during tissue development are covered.Methods of biomaterial scaffold fabrication are compared and contrasted. Cell culture and laboratory safety, cell kinetics, mass transfer in tissue and bioreactor are covered. Differentiation of cell specific lineages from stem cells and ethical issues in tissue engineering are discussed based on cased studies. This course addresses potential clinical impact of tissue engineering and its future direction.